Virus:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Infection:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Cell Culture:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Recombinant:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Protease Inhibitor:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Electron Microscopy:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Transfection:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Reverse Transcription:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Real-time Polymerase Chain Reaction:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Bradford Protein Assay:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Fluorescence In Situ Hybridization:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
Hybridization:Article Title: Molecular mechanisms of stress-induced reactivation in mumps virus condensates.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-MuV-N protein (clone 8H4) Abcam Cat#ab9876; RRID:AB_296684 Goat anti-mouse IgG secondary antibody Alexa Fluor 488 (highly cross-adsorbed) Invitrogen Cat#A-11029; RRID:AB_2534088 Goat anti-mouse IgG secondary antibody Alexa Fluor plus 647 (highly cross-adsorbed) Invitrogen Cat#A-32728; RRID:AB_2633277 Mouse monoclonal anti-GAPDH (clone 6C5) Santa Cruz Cat#sc-32233; RRID:AB_627679 Goat anti-mouse IgG (H/L):HRP (multi species absorbed) Bio-Rad Cat#STAR117P; RRID:AB_323839 Bacterial and virus strains Mumps virus Enders strain Accidental infection in the HeLa cell culture GenBank: GU980052 Single nucleotide variations from Enders strain are reported below Chemicals, peptides, and recombinant proteins Sodium arsenite Sigma-Aldrich S7400; CAS: 7784-46-5 Potassium arsenate Sigma-Aldrich A6631; CAS: 7784-41-0 1,6-Hexanediol Sigma-Aldrich 240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI Cell Signaling Technology Cat# 8961 Oligo d(T)23 primer New England Biolabs Cat#S1327S EDTA-free protease inhibitor cocktail Roche 04693132001 PhosSTOP Phosphatase inhibitor cocktail Roche 4906845001 Benzonase Nuclease, ultrapure Millipore E8263; CAS: 9025-65-4 Ribonuclease inhibitor Sigma-Aldrich R1158 Heparin sodium Sigma-Aldrich H3149; 9041-08-1 Protein-A/Gold, EM-grade 10 nm Electron Microscopy Sciences Cat#50-281-97 1-mm crimson beads (FluoSpheres carboxylate-modified microspheres, 625/645) Invitrogen Cat#F8816 Critical commercial assays jetPRIME Transfection Reagent Polyplus Ref: 101000027 RNeasy Micro Kit Qiagen Cat#74004 ProtoScript II Reverse Transcriptase Kit New England Biolabs Cat#M0368 TaqPath qPCR Master Mix, CG Applied Biosystems Cat#A15297 RNA Nano 6000 Assay Kit Agilent Technologies Cat#5067-1511 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 SPRIselect beads Beckman Coulter B23318 DNA High Sensitivity kit Agilent Technologies 5067-4626 Qubit dsDNA High Sensitivity kit Invitrogen Cat#Q32851 Bradford Protein Assay Dye Bio-Rad Cat#5000006 Stellaris RNA FISH Wash Buffer A Biosearch Technologies Cat#SMF-WA1-60 Stellaris RNA FISH Hybridization Buffer Biosearch Technologies Cat#SMF-HB1-10 Stellaris RNA FISH Wash Buffer B Biosearch Technologies Cat#SMF-WB1-20 GFP-Trap magnetic agarose ChromoTek Cat#gtma-20 Deposited data PIV5 nucleocapsid-RNA complex Alayyoubi et al.52 PDB: 4XJN PIV5 L-P complex Abdella et al.46 PDB: 6V85 (Continued on next page) Cell 186, 1877–1894.e1–e15, April 27, 2023 e1
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